Indoor Season |
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| Year | Name | Institution | Time |
| 1998 | Keely Subin | Rhode Island College | 27.43 |
| 1999 | Crissy McCullah | Rhode Island College | 27.28 |
| 2000 | Crissy McCullah | Rhode Island College | 27.28 |
| 2001 | Lauren McPartlin | Southern Maine | 28.09 |
| 2002 | Summer Sage-Williams | Eastern Connecticut | 26.55 |
| 2003 | Kim Brewer | Southern Maine | 27.14 |
| 2004 | Dana Skorupa | Rhode Island College | 26.58 |
| 2005 | Dana Skorupa | Rhode Island College | 26.78 |
| 2006 | Dana Skorupa | Rhode Island College | 26.80 |
| 2007 | Emily Artsani | Southern Maine | 27.06 |
| 2008 | Emily Artsani | Southern Maine | 26.69 |
| 2009 | Emily Artsani | Southern Maine | 27.09 |
| 2010 | Emily Artsani | Southern Maine | 26.94 |
| 2011 | Jillian Van Ness | UMass Dartmouth | 27.05 |
| 2012 | Hannah Wiley | Southern Maine | 26.03 |
| 2013 | Jaimee Dorsey | Rhode Island College | 25.96 |
| 2014 | Hulerie McGuffie | UMass Boston | 25.81 |
| 2015 | Autumn Wosencroft | Rhode Island College | 26.05 |
| 2016 | Hulerie McGuffie | UMass Boston | 25.72 |
| 2017 | Wadeline Jonathas | UMass Boston | 25.15 |
| 2018 | Wadeline Jonathas | UMass Boston | 24.93 |
| 2019 | Emma Landroche | Rhode Island College | 26.23 |
Outdoor Season |
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| Year | Name | Institution | Time |
| 1998 | Laura Caselden | UMass Dartmouth | 27.63 |
| 1999 | Keely Subin | Rhode Island College | 27.33 |
| 2000 | Dapheney Jean-Baptiste | Rhode Island College | 26.68 |
| 2001 | Summer Sage-Williams | Eastern Connecticut | 27.34 |
| 2002 | Nicole Geyselaers | Keene State | 26.37 |
| 2003 | Kate McCalligett | Rhode Island College | 26.25 |
| 2004 | Dana Skorupa | Rhode Island College | 25.72 |
| 2005 | Dana Skorupa | Rhode Island College | 26.48 |
| 2006 | Dana Skorupa | Rhode Island College | 26.27 |
| 2007 | Emily Artesani | Southern Maine | 26.13 |
| 2008 | Emily Artesani | Southern Maine | 26.32 |
| 2009 | Emily Artesani | Southern Maine | 26.14 |
| 2010 | Emily Artesani | Southern Maine | 26.94 |
| 2011 | Jillian VanNess | UMass Dartmouth | 26.93 |
| 2012 | Jillian VanNess | UMass Dartmouth | 25.45 |
| 2013 | Jaimee Dorsey | Rhode Island College | 25.23 |
| 2014 | Hulerie McGuffie | UMass Boston | 25.21 |
| 2015 | Hulerie McGuffie | UMass Boston | 25.45 |
| 2016 | Hulerie McGuffie | UMass Boston | 25.09 |
| 2017 | Wadeline Jonathas | UMass Boston | 23.79 |
| 2018 | Wadeline Jonathas | UMass Boston | 23.99 |